If you wish to use RNAi to reduce the expression of a gene in an organism. What would you inject into the organism?
A) Dicer with double- stranded (ds) DNA
B) dsRNA including transposase
C) ssRNA complementary to cDNA
D) dsRNA homologous to the gene
E) ssRNA complementary to introns of the target gene

Question

Quizplus · Accepted Answer

dsRNA homologous to the gene is used in RNA interference (RNAi) to reduce the expression of a specific gene. When introduced into the organism, the dsRNA is processed into small interfering RNAs (siRNAs) by the enzyme Dicer. These siRNAs then guide the RNA-induced silencing complex (RISC) to the target mRNA, leading to its degradation and thus reducing gene expression.

If You Wish to Use RNAi to Reduce the Expression