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Identifying an Autosomal Recessive Mutation in a Mutagenic Screen in Drosophila

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Identifying an autosomal recessive mutation in a mutagenic screen in Drosophila requires identification of a mutant in the F3 generation. If testing for a sex- linked recessive lethal mutation (e.g., cn l + using a balancer chromosome such as cnCyO), in which generation can lines with mutations be identified?

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