What technique would you use to detect a target sequence in an intact chromosome using a labeled molecular probe?
A) karyotyping
B) DNA sequencing
C) polymerase chain reaction (PCR)
D) fluorescent in situ hybridization (FISH)
E) western/immuno blotting

Question

Quizplus · Accepted Answer

Fluorescent in situ hybridization (FISH) is a technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. It uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity. This allows for the visualization of the target sequences in situ, meaning within the intact chromosomes in the cell.

What Technique Would You Use to Detect a Target Sequence