Question 1

Using the Sanger method of DNA sequencing, results in the figure below are obtained following gel electrophoresis of the DNA fragments.  A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?

Accepted Answer

The answer of Using the Sanger method of DNA sequencing,...

Question 2

Because of its antiparallel nature, DNA is often referred to as a .

Accepted Answer

The answer of Because of its antiparallel nature, DNA is...

Question 3

An example of a purine is

Accepted Answer

Guanine is a purine, which is a type of nitrogenous base found in DNA and RNA.

Question 4

The specific field of study that examines the gene products of a genome is known as

Accepted Answer

Proteomics is the field of study that examines the gene products, which are proteins, of a genome.

Question 5

Which of the following shows Chargaff's equivalence?

Accepted Answer

Chargaff's equivalence refers to the base pairing rule in double-stranded DNA where the amount of adenine (A) equals thymine (T) and the amount of cytosine (C) equals guanine (G). In synthetic DNA with one strand all A and the other all T, this rule is satisfied.

Question 6

demonstrated that there were equivalent amounts of adenine and thymine, and equivalent amounts of guanine and cytosine.

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The answer of demonstrated that there were equivalent amounts of...

Question 7

Base sequence variations between individuals that result from point mutations are called

Accepted Answer

Single nucleotide polymorphisms (SNPs) are variations in a single nucleotide that occur at a specific position in the genome, and are the most common type of genetic variation among people.

Question 8

A variety of E. coli clones have been isolated, the DNA has been extracted, digested with restriction enzymes, denatured, separated by gel electrophoresis, and transferred to nylon membrane by the Southern blot procedure. A radioactive probe is used that hybridizes with a gene of interest. The DNA corresponding to a single clone yields a single DNA band after probing the blot. Based on this information only,

Accepted Answer

The answer of A variety of E. coli clones have...

Question 9

With which organelle membrane would you expect to find the receptor protein importin?

Accepted Answer

Importin is a receptor protein that facilitates the transport of proteins into the nucleus by recognizing nuclear localization signals and interacting with the nuclear pore complex.

Question 10

You are designing, by genetic engineering, a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell. To accomplish this one could add

Accepted Answer

A nuclear localization signal (NLS) is a sequence of amino acids that 'tags' a protein for import into the nucleus by nuclear transport. Adding an NLS to the DNA ensures it will be present in the mature protein, directing it to the nucleus.

Question 11

DNA isolated from cow liver cells contains 28% A; what percent will be C?

Accepted Answer

According to Chargaff's rules, in DNA, the amount of adenine (A) equals thymine (T), and the amount of cytosine (C) equals guanine (G). If A is 28%, then T is also 28%. This leaves 44% for C and G combined, so C is 22%.

Question 12

Which of the following is the correct order of the levels of DNA packaging in eukaryotic chromosomes?

Accepted Answer

The correct order of the levels of DNA packaging in eukaryotic chromosomes is: nucleosome -chromatin fiber -looped domains -heterochromatin.

Question 13

Which one of the following is NOT characteristic of prokaryotic genomes?

Accepted Answer

Prokaryotic genomes typically do not contain introns; introns are more characteristic of eukaryotic genomes.

Question 14

Common SNPs may be important in causing in humans or in of disorders.

Accepted Answer

The answer of Common SNPs may be important in causing...

Quiz 18: The Structural Basis of Cellular Information: DNA, Chromosomes, and the Nucleus

Using the Sanger method of DNA sequencing, results in the figure below are obtained following gel electrophoresis of the DNA fragments. A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?