The concentrations of enzymes are determined by using pseudo first-order conditions in which
A) S concentration is kept the same and the rate is measured.
B) E concentration is kept the same and the rate is measured.
C) S concentration is constant, E concentration varied and the rate is measured.
D) E concentration is constant, S concentration varied and the rate is measured.
E) All of the above

Question

Quizplus · Accepted Answer

In pseudo first-order conditions, the substrate (S) concentration is kept constant while the enzyme (E) concentration is varied to measure the rate, allowing the reaction to appear first-order with respect to the enzyme.

The Concentrations of Enzymes Are Determined by Using Pseudo First-Order