Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction? A) Measure K_min the presence of one substrate at a time. B) Measure the rate with respect to one substrate while varying the concentration of another substrate. C) Measure the rate while adding inactivators for all but one substrate. D) Any of the above

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Measuring the rate with respect to one substrate while varying the concentration of another substrate can help distinguish between different kinetic mechanisms by revealing how substrates interact with the enzyme and each other.

Which Might Be a Method Used for Distinguishing Among Several