The recognition site of some restriction enzymes are shown. Which will produce sticky ends?
A) EcoRI G↓AATTC
B) SmaI CCC↓GGG
C) XhoI C↓TCGAG
D) both EcoRI and XhoI
E) All the enzymes shown will leave sticky ends.

Question

Quizplus · Accepted Answer

EcoRI and XhoI will produce sticky ends because their recognition sites are palindromic and they cut the DNA at different locations on the two strands, leaving single-stranded overhangs.

The Recognition Site of Some Restriction Enzymes Are Shown