Alkaline hydrolysis of RNA produces a mixture of 2ʹ and 3ʹ

Name: Alkaline hydrolysis of RNA produces a mixture of 2ʹ and 3ʹ - nucleotides. Hydrolysis by RNase A produces on the 3ʹ-nucleotides.
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Question

Alkaline hydrolysis of RNA produces a mixture of 2ʹ and 3ʹ - nucleotides. Hydrolysis by RNase A produces on the 3ʹ-nucleotides.

Quizplus · Accepted Answer

RNase A specifically cleaves RNA at the 3' end of pyrimidine nucleotides, resulting in 3'-nucleotides, whereas alkaline hydrolysis randomly cleaves the phosphodiester bonds, producing a mixture of 2' and 3'-nucleotides.