Which technique can be used for molecular weight (mass)determinations in proteins?
A)X- ray diffraction
B)chromatofocusing
C)diethylaminoethyl (DEAE)cellulose chromatography
D)affinity chromatography
E)SDS-PAGE

Question

Quizplus · Accepted Answer

SDS-PAGE can be used for determining the molecular weight of proteins by separating them based on their size and charge. The proteins are denatured by SDS (sodium dodecyl sulfate) and loaded onto a polyacrylamide gel, which is then subjected to electrophoresis. The proteins move through the gel based on their size, with smaller proteins migrating farther. A standard ladder of known molecular weights can be run alongside the protein sample, allowing for the determination of the molecular weight of the proteins of interest.

Which Technique Can Be Used for Molecular Weight (Mass)determinations in Proteins