How are fragments already in a mass spectrometer further fragmented?
A)injection of pepsin
B)injection of more trypsin
C)collision of peptides with an inert gas
D)collision of peptides with nitrogen gas
E)collision of peptides with oxygen

Question

Quizplus · Accepted Answer

Fragments in a mass spectrometer are further fragmented through collision with an inert gas, such as helium or argon. This process is known as collision-induced dissociation (CID) or collision-activated dissociation (CAD). The collisions cause the already fragmented ions to undergo further fragmentation, generating smaller fragments that can be analyzed to obtain more structural information.

How Are Fragments Already in a Mass Spectrometer Further Fragmented