What is the result of treating the full complement of genomic DNA with low concentrations of restriction enzymes under conditions in which most of the susceptible sites are not cleaved?
A)The genomic DNA is fragmented into a defined and limited set of fragments.
B)The genomic DNA is fully degraded.
C)The genomic DNA is barely damaged.
D)The genomic DNA is cut into random fragments of many different sizes.
E)The genomic DNA is fragmented into a small set of very large pieces of DNA.

Question

Quizplus · Accepted Answer

Treating the full complement of genomic DNA with low concentrations of restriction enzymes under conditions in which most of the susceptible sites are not cleaved results in random fragments of many different sizes. This is because the restriction enzymes will cleave at the few sites where they are most efficient, and leave many other sites uncut. Thus, the resulting fragments will be of variable sizes.

What Is the Result of Treating the Full Complement of Genomic