Which enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?
A)RNase H
B)reverse transcriptase
C)DNA polymerase I
D)RNase K
E)DNA ligase

Question

Quizplus · Accepted Answer

DNA polymerase I is used to replace the RNA strand of a DNA-RNA hybrid with DNA during the preparation of a cDNA library, as it has both polymerase and exonuclease activities.

Which Enzyme Is Used to Replace the RNA of a DNA-RNA