What is used to separate DNA fragments in the cycle sequencing method of DNA sequencing in such a way that chains differing by only one nucleotide in length can be separated?
A)SDS-PAGE
B)non-denaturing PAGE
C)agarose gel electrophoresis
D)electrophoresis on very thin capillary gels
E)gel filtration chromatography

Question

Quizplus · Accepted Answer

Electrophoresis on very thin capillary gels is used in cycle sequencing method of DNA sequencing to separate DNA fragments in such a way that chains differing by only one nucleotide in length can be separated. Capillary electrophoresis allows for higher resolution separation due to the high efficiency of the capillaries, resulting in better separation of closely related DNA fragments.

What Is Used to Separate DNA Fragments in the Cycle