The CRISPR-cas9 system uses _______ as a template to locate and cleave specific___________ targets.
A)siRNA, DNA
B)siRNA, RNA
C)dsDNA, RNA
D)sgRNA, RNA
E)sgRNA, DNA

Question

Quizplus · Accepted Answer

The CRISPR-cas9 system uses a single guide RNA (sgRNA) as a template to locate and cleave specific DNA targets. siRNA is a separate RNA interference system that operates in the cytoplasm and targets mRNA, not DNA. dsDNA refers to double-stranded DNA as a molecule, but does not specify a system or mechanism. RNA is not used as a template for locating and cleaving DNA by the CRISPR-cas9 system.

The CRISPR-Cas9 System Uses _______ as a Template to Locate