A scientist wishes to visualize movement of a protein in a living cell. Which procedure would work best?
A)pulse-chase
B)fusion of the green fluorescent protein gene to the protein that is to be tracked through the cell
C)fusion of the green fluorescent protein gene to the gene encoding the protein to be tracked through the cell
D)pulse-chase using fluorescent antibodies
E)all of these would work well

Question

Quizplus · Accepted Answer

Fusion of the green fluorescent protein (GFP) gene to the gene encoding the protein of interest allows for the direct visualization of the protein's movement within living cells. This method does not require the cell to be fixed or killed, making it ideal for live-cell imaging.

A Scientist Wishes to Visualize Movement of a Protein in a Living