DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from a gel to a nitrocellulose paper?
A) to attach the DNA fragments to a permanent substrate
B) to separate the two complementary DNA strands
C) to transfer only the DNA that is of interest
D) to prepare the DNA for digestion with restriction enzymes
E) to separate out the PCRs

Question

Quizplus · Accepted Answer

Transferring the DNA fragments from a gel to a nitrocellulose paper helps to permanently attach the DNA fragments to a substrate which allows them to be probed with a labeled probe. This process is called Southern blotting and allows for easy identification and analysis of specific DNA sequences.

DNA Fragments from a Gel Are Transferred to a Nitrocellulose