A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated

Question

Quizplus · Accepted Answer

Recombination resulting in replacement of the wild type with the mutated gene is necessary in order to disable the function of the gene. This would require the mutated sequence to replace the original sequence in the cell's genome. Choices B, C, D, and E are not directly related to this process.

A Researcher Has Used in Vitro Mutagenesis to Mutate a Cloned