Which is the key to the chain termination method of DNA sequencing?

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Quizplus · Accepted Answer

2',3'-dideoxynucleoside triphosphate is the key to the chain termination method of DNA sequencing. It terminates the DNA chain because it lacks the necessary hydroxyl group at the 3' carbon to form a phosphodiester bond with the next nucleotide, therefore stopping further chain elongation. The termination occurs randomly at any point along the DNA fragment, resulting in a set of fragments of different sizes that can be separated on a polyacrylamide gel to determine the DNA sequence. Restriction endonucleases and a primer are not key to the chain termination method of DNA sequencing.

Which Is the Key to the Chain Termination Method of DNA