The phenomenon that allows a quencher dye to reduce the fluorescence of the reporter dye in real-time PCR techniques is called

Question

Quizplus · Accepted Answer

The phenomenon described in the question is called fluorescence resonance energy transfer (FRET), which involves the transfer of energy from a fluorescent molecule (the reporter dye) to a nearby non-fluorescent molecule (the quencher dye). As the reporter dye emits light, the quencher dye absorbs the energy and dissipates it as heat, effectively quenching the fluorescence of the reporter dye. This process can be used in real-time PCR techniques to monitor the amplification of DNA by measuring the decrease in fluorescence caused by the quenching of the reporter dye.

The Phenomenon That Allows a Quencher Dye to Reduce the Fluorescence