In the first stage of the western blot procedure, a technique known as SDS-PAGE is used to separate proteins according to their

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SDS-PAGE separates proteins based on their molecular weights. This is done by using a gel matrix with pores that allow smaller proteins to move faster through the gel, while larger proteins move slower. Additionally, SDS (sodium dodecyl sulfate) is used to denature the proteins and give them a negative charge, further facilitating separation based on size.

In the First Stage of the Western Blot Procedure, a Technique