Real-time PCR can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

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Real-time PCR requires cDNA as a template, so RNA must first be converted into cDNA through a process called reverse transcription (RT). This step is commonly performed using reverse transcriptase enzymes and specific primers that anneal to the RNA template. Once the cDNA is generated, it can be used as a template for the real-time PCR reaction to quantify the original amount of RNA. The other options listed are not necessary for this application.

Real-Time PCR Can Be Used to Quantify the Original Amount