In the laboratory assay on digestion of the protein BAPNA by trypsin, what was the purpose of including a tube with trypsin alone?
A) to indicate that trypsin did not contribute to the clear (positive) color
B) to indicate that BAPNA did not contribute to the yellow (positive) color
C) to indicate that trypsin did not contribute to the yellow (positive) color
D) to indicate that BAPNA did not contribute to the clear (positive) color

Question

Quizplus · Accepted Answer

The purpose of including a tube with trypsin alone in the assay is to demonstrate that trypsin itself does not produce the yellow color, indicating that any observed color change in the experiment is due to the interaction between trypsin and BAPNA, not trypsin alone.

In the Laboratory Assay on Digestion of the Protein BAPNA