Use the following information to answer the question.
In an experiment, DNA from the linear form of the bacteriophage Lambda was cut into fragments using the restriction enzyme Hind III. Hind III cuts the Lambda DNA between the adenine nucleotides on the complimentary strands in a specific sequence, as indicated in the diagram, producing eight different size fragments. These fragments are then separated with an electrical current based on size after the DNA fragments are placed in a porous gel, a process called gel electrophoresis.
cut site
T TCGAA 5'
cut site
Select an observation that best describes a correct aspect of the two processes of restriction
Digest and gel electrophoresis.
A) When separated on a gel, the pattern of DNA bands will be characteristic of those cut with Hind III; different restriction enzymes will not produce these same fragments.
B) The sequence 5′-AAGCTT-3′ is found eight times in the Lambda genome, and the restriction enzyme Hind III finds each location.
C) If an electrical current is not used, eight separate DNA bands would be visible, but they would not be separated as much as when an electrical current is used.
D) Only the restriction enzyme Hind III can be used to cut Lambda DNA since restriction enzymes are specific to the type of DNA they can cut.
Correct Answer:
Verified
Q22: Scientists developed a set of guidelines to
Q29: Which of the following problems with animal
Q48: Which of the following characteristics of short
Q49: DNA technology has many medical applications. Which
Q50: Plants are more readily manipulated by genetic
Q52: Why might using retroviral vectors for gene
Q53: Transgenic mice are useful to human researchers
Q53: Which of the following sequences in double-stranded
Q54: Use the following information to answer the
Q56: One predicted aspect of climate change is
Unlock this Answer For Free Now!
View this answer and more for free by performing one of the following actions
Scan the QR code to install the App and get 2 free unlocks
Unlock quizzes for free by uploading documents