What makes eukaryotic transcripts easier to isolate than bacterial transcripts?
A) Eukaryotic transcripts are not methylated but their genes are often methylated.
B) Larger transcript size in eukaryotes enables easy size-selection methods.
C) mRNA is polyadenylated in eukaryotes.
D) Transcripts are the most abundant RNAs in eukaryotes.

Question

Quizplus · Accepted Answer

mRNA in eukaryotes have a poly-A tail at their 3' end, making them easier to isolate using poly-T oligonucleotide probes. Bacterial mRNA lacks this tail, making it more difficult to isolate.

What Makes Eukaryotic Transcripts Easier to Isolate Than Bacterial Transcripts