Green fluorescent protein (GFP) is used for detecting translational activity of a fused protein, whereas lacZ reporters are used to detect transcriptional activity of a fused gene.

Question

Quizplus · Accepted Answer

Green fluorescent protein (GFP) is actually used for detecting expression of a fused gene, as it allows visualization of the protein in living cells. On the other hand, lacZ reporters are used to detect the activity of a promoter by measuring the expression of the lacZ gene, which encodes for beta-galactosidase.

Green Fluorescent Protein (GFP) Is Used for Detecting Translational Activity