One method to circumvent issues with introns when expressing a eukaryotic gene in a bacterium is to simply clone the mature transcript.

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Cloning only the mature transcript excludes the introns, which are non-coding regions in eukaryotic genes that must be removed by splicing before translation can occur. Bacteria lack the necessary splicing machinery to process introns, so including them in the cloned gene could lead to incomplete or incorrect protein production. By cloning only the mature transcript, the gene can be expressed more easily and accurately in the bacterial host.

One Method to Circumvent Issues with Introns When Expressing a Eukaryotic