Indirect selection
A) is necessary to isolate auxotrophic mutants.
B) uses media on which the mutant but not the parental cell type will grow.
C) uses media that reverses the mutation.
D) uses media upon which neither the parental cell type nor mutant grows.
E) is necessary to isolate his⁺ prototrophs.

Question

Quizplus · Accepted Answer

Indirect selection is necessary to isolate auxotrophic mutants because it involves identifying mutants that cannot grow on minimal media without specific supplements, indicating a loss of function mutation.

Indirect Selection