Replica plating
A) is useful for direct selection of antibiotic resistance AND is useful for identifying auxotrophs.
B) uses media on which the mutant will grow but the parental cell type will not AND is useful for identifying prototrophs.
C) is useful for identifying auxotrophs AND uses media on which the mutant will not grow but the parental cell type will.
D) is useful for identifying auxotrophs AND uses media on which the mutant will grow but the parental cell type will not.
E) is useful for direct selection of antibiotic resistance AND is useful for identifying prototrophs.

Question

Replica plating
A) is useful for direct selection of antibiotic resistance AND is useful for identifying auxotrophs.
B) uses media on which the mutant will grow but the parental cell type will not AND is useful for identifying prototrophs.
C) is useful for identifying auxotrophs AND uses media on which the mutant will not grow but the parental cell type will.
D) is useful for identifying auxotrophs AND uses media on which the mutant will grow but the parental cell type will not.
E) is useful for direct selection of antibiotic resistance AND is useful for identifying prototrophs.

Quizplus · Accepted Answer

Replica plating is a technique used to identify auxotrophs by transferring colonies from a master plate to a new plate with selective media. Auxotrophs will not grow on media lacking the nutrient they cannot synthesize, while the parental (prototrophic) cells will grow.

Replica Plating