Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are two primers (a forward and a reverse) necessary?
A) Yes-you can't perform PCR without two specific primers to amplify the region in question in the DNA.
B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer.
C) No-you actually need a primer pair for each round of DNA amplification ... so you'll need many, many primer pairs, depending how many rounds you plan to do.
D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process.
E) Yes-if you only have one primer, you will only be able to determine the dideoxynucleotide sequence of RNA, which is a single-stranded molecule.

Question

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are two primers (a forward and a reverse) necessary?
A) Yes-you can't perform PCR without two specific primers to amplify the region in question in the DNA.
B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer.
C) No-you actually need a primer pair for each round of DNA amplification ... so you'll need many, many primer pairs, depending how many rounds you plan to do.
D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process.
E) Yes-if you only have one primer, you will only be able to determine the dideoxynucleotide sequence of RNA, which is a single-stranded molecule.

Quizplus · Accepted Answer

For DNA sequencing, particularly when using the Sanger method (dideoxynucleotide sequencing), it is possible to use only one primer (either forward or reverse) for the reaction. This primer allows for the synthesis of DNA strands of varying lengths, which can then be separated by size to determine the DNA sequence. Two primers are not necessary for this process as the sequencing reaction is designed to read the sequence in one direction from the point of primer attachment.

Polymerase Chain Reaction (PCR) Can Be Used to Perform DNA