Scientists have cloned the human insulin gene into E. coli. Which of the following would NOT have been used in the procedure?
A) A plasmid
B) Competent bacteria
C) A restriction enzyme
D) DNA ligase
E) A bacteriophage

Question

Quizplus · Accepted Answer

A bacteriophage would not have been used in the procedure. The other choices are commonly used in gene cloning techniques: A plasmid is used as a vector to carry the gene of interest; competent bacteria are used to take up the vector with the cloned gene; a restriction enzyme is used to cut the plasmid and insert the gene; and DNA ligase is used to seal the plasmid back together with the inserted gene.

Scientists Have Cloned the Human Insulin Gene into E