Why are primers needed in DNA sequencing?
A) DNA polymerase needs a primer because it can only add nucleotides to an existing nucleotide strand.
B) DNA polymerase needs a primer to help it recognize a vector for gene insertion.
C) DNA polymerase needs a primer for proofreading and mismatch repair after a gene has been sequenced.
D) The primer acts as a template for the dideoxynucleotides and deoxynucleotides.
E) All of the answer choices are correct.

Question

Why are primers needed in DNA sequencing?
A) DNA polymerase needs a primer because it can only add nucleotides to an existing nucleotide strand.
B) DNA polymerase needs a primer to help it recognize a vector for gene insertion.
C) DNA polymerase needs a primer for proofreading and mismatch repair after a gene has been sequenced.
D) The primer acts as a template for the dideoxynucleotides and deoxynucleotides.
E) All of the answer choices are correct.

Quizplus · Accepted Answer

DNA polymerase can only add nucleotides to an existing nucleotide strand, so a primer is necessary to start the sequencing process. The other options are not accurate.

Why Are Primers Needed in DNA Sequencing