Which is the CORRECT definition of PCR?
A) Use of the enzyme reverse transcriptase to generate cDNA from mRNA, and then amplifying the cDNA exponentially using RNA polymerase.
B) In vitro process of determining the nucleotide sequence of a DNA molecule using a variety of enzymes.
C) In vitro technique used to repeatedly duplicate (amplify) a specific region of a DNA molecule, increasing the number of copies exponentially.
D) Procedure in which a fragment of DNA is inserted into a vector and then transferred into another cell, where it then replicates.
E) The process in which two complementary strands of DNA from different sources are annealed to create a hybrid double-stranded molecule.

Question

Which is the CORRECT definition of PCR?
A) Use of the enzyme reverse transcriptase to generate cDNA from mRNA, and then amplifying the cDNA exponentially using RNA polymerase.
B) In vitro process of determining the nucleotide sequence of a DNA molecule using a variety of enzymes.
C) In vitro technique used to repeatedly duplicate (amplify) a specific region of a DNA molecule, increasing the number of copies exponentially.
D) Procedure in which a fragment of DNA is inserted into a vector and then transferred into another cell, where it then replicates.
E) The process in which two complementary strands of DNA from different sources are annealed to create a hybrid double-stranded molecule.

Quizplus · Accepted Answer

PCR (Polymerase Chain Reaction) is an in vitro technique used to repeatedly duplicate (amplify) a specific region of a DNA molecule, increasing the number of copies exponentially. This process involves the use of a heat-stable DNA polymerase enzyme, specific primers, and repeated cycles of heating and cooling to denature, anneal, and extend the DNA strands. PCR is widely used in various fields of research, including genetics, forensics, and medical diagnostics.

Which Is the CORRECT Definition of PCR