During PCR, which primer anneals to template DNA at its 3' end?
A) The forward primer only.
B) The reverse primer only.
C) Either the forward primer or the reverse primer.
D) Both the forward primer and the reverse primer.
E) Primers don't bind to template DNA.

Question

During PCR, which primer anneals to template DNA at its 3' end? 
A) The forward primer only.
B) The reverse primer only.
C) Either the forward primer or the reverse primer.
D) Both the forward primer and the reverse primer.
E) Primers don't bind to template DNA.

Quizplus · Accepted Answer

Both the forward and reverse primers anneal to their respective complementary sequences on the template DNA at their 3' ends during PCR. This is essential for DNA polymerase to extend the primers and synthesize the new DNA strand.

During PCR, Which Primer Anneals to Template DNA at Its