Question 1

To dilute a serum specimen for a 1:10 dilution
-Serologic pipette

Accepted Answer

Serologic pipettes are marked with the letters TD (to deliver), indicating that they are designed to deliver a specific volume of liquid, with the volume being the amount contained between two calibration marks on the pipette. This is essential for creating precise dilutions, such as a 1:10 dilution of a serum specimen.

Question 2

Match the term with the appropriate definition.
-Chyle

Accepted Answer

Chyle refers to an emulsion of fat globules that often appears in serum after eating, during digestion. It is a milky bodily fluid consisting of lymph and emulsified fats, absorbed by the lymphatic system from the small intestine during digestion.

Question 3

A dilution is a(n)

Accepted Answer

B

Question 4

Complement can be inactivated in human serum by heating to _____ $\degree$ C.

Accepted Answer

Complement proteins in human serum can be inactivated by heating to 56 degrees Celsius, which is a common laboratory procedure to prevent complement-mediated activities in experiments.

Question 5

Automatic pipettes have the advantage of

Accepted Answer

Automatic pipettes are designed to be fast, allow for repetitive measurement of solutions, and deliver equal volumes of solutions, making all of the options correct.

Question 6

A specimen should be reinactivated when more than ______ hour(s) has (have) elapsed since inactivation.

Accepted Answer

Inactivation refers to the process of rendering a specimen (such as a virus, bacteria, or other microorganisms) non-infectious or non-viable. The specific time frame for reactivation depends on the protocol or guidelines being followed, but a common standard is to consider reactivation if more than 4 hours have elapsed since inactivation. This is because the effectiveness of the inactivation process can decrease over time, potentially allowing the specimen to regain viability or infectiousness if not properly handled.

Question 7

To dilute a serum specimen for a 1:10 dilution
-parts of distilled water would be added to the 10-mL mark.

Accepted Answer

The answer of To dilute a serum specimen for a...

Question 8

A central concept of serologic testing is

Accepted Answer

The answer of A central concept of serologic testing is
...

Question 9

To dilute a serum specimen for a 1:10 dilution
-parts of serum would be added to a tube, and then

Accepted Answer

1 part of serum is added to 9 parts of diluent to make a 1:10 dilution.

Question 10

A graduated pipette can be used when

Accepted Answer

Graduated pipettes are used when great accuracy is not needed, as they are less precise than volumetric pipettes.

Question 11

Match the term with the appropriate definition.
-Hemolysis

Accepted Answer

Hemolysis refers to the rupturing of red blood cells, which releases hemoglobin into the surrounding fluid (plasma or serum), giving it a pink or reddish color.

Question 12

Factors that can denature, coagulate, or alter protein molecules include

Accepted Answer

The answer of Factors that can denature, coagulate, or alter...

Question 13

To dilute a serum specimen for a 1:10 dilution
-Graduated pipette

Accepted Answer

A graduated pipette delivers a specific volume of liquid, as indicated by the calibration marks on the pipette.

Question 14

A meniscus is the

Accepted Answer

A meniscus is the curve seen at the top surface of a liquid in response to its container. This curvature is due to the liquid's surface tension and the adhesive forces between the liquid and container.

Question 15

Match the term with the appropriate definition.
-Icteric

Accepted Answer

Icteric refers to a yellowish coloration, which is typically associated with jaundice or increased bilirubin levels in the plasma or serum.

Question 16

Serum for the detection of antibodies should be drawn during the

Accepted Answer

Serum for the detection of antibodies should be drawn during both the acute and convalescent phases of illness. This allows for the comparison of antibody levels to determine if there has been a significant rise in antibody titers, indicating a recent infection.

Question 17

The term antibody titer is defined as

Accepted Answer

The term antibody titer refers to the highest dilution of a patient's serum that still produces a positive reaction in a specific test, essentially measuring the concentration of antibodies present.

Quiz 8: Basic Serologic Laboratory Techniques

To dilute a serum specimen for a 1:10 dilution -Serologic pipette

Match the term with the appropriate definition. -Chyle

A dilution is a(n)

Complement can be inactivated in human serum by heating to _____ °\degree° C.

Automatic pipettes have the advantage of

A specimen should be reinactivated when more than ______ hour(s) has (have) elapsed since inactivation.

To dilute a serum specimen for a 1:10 dilution -parts of distilled water would be added to the 10-mL mark.

A central concept of serologic testing is

To dilute a serum specimen for a 1:10 dilution -parts of serum would be added to a tube, and then

A graduated pipette can be used when

Match the term with the appropriate definition. -Hemolysis

Factors that can denature, coagulate, or alter protein molecules include

To dilute a serum specimen for a 1:10 dilution -Graduated pipette

A meniscus is the

Match the term with the appropriate definition. -Icteric

Serum for the detection of antibodies should be drawn during the

The term antibody titer is defined as

Complement can be inactivated in human serum by heating to _____ $\degree$ C.