Question 1

The system of taxonomy used today was originated by (Linnaeus/Darwin/Woese). (Be sure to capitalize your answer.)

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Question 2

Compare and contrast the three domains identified by Woese and Fox: Eukarya, Bacteria, and Archaea.

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Question 3

The total magnification using a 10 ocular and a 100 objective would be (110/1000/10000)×. (Be sure your answer is a numeral.)

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Question 4

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron) microscopy.

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Question 5

You are a scientist studying the highly specific interactions of bacteriophages with their host cells when they first encounter the cell. Discuss what microscope(s) and preparation procedures you might use for this study.

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Question 6

A serological test that involves the clumping of antigen and antibody is the (agglutination/antigen/ELISA) test.

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Question 7

In the Schaffer-Fulton endospore stain, heat is a (fixation/mordant/staining) step.

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Question 8

A primary purpose for the use of stains in microscopy is to increase the (magnification/brightness/contrast) of a specimen.

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Question 9

List and explain five types of techniques that can be used to identify unknown microorganisms.

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Question 10

The part of the microscope indicated by the arrow in Figure 4.1 is the (ocular/objective/condenser) lens.

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The part of the microscope indicated...

Question 11

The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).

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Question 12

Figure 4.2 represents a (dichotomous/classification/taxonomic) key of the type used to identify a microbe.

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Figure 4.2 represents a (dichotomous/classification/taxonomic) key...

Question 13

PCR is a method for identifying microbes based on their (antigens/genes/morphology).

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Question 14

Carl Woese and George Fox proposed the (phylum/domain/family), a taxon that contains multiple kingdoms.

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Question 15

A (decolorizer/mordant/fixer) is a substance that binds to a dye and makes it less soluble.

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Question 16

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

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Question 17

Compare and contrast the light microscope with the electron microscope.

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Question 18

A(n) (acidic/metallic/fluorescent) molecule is one that absorbs invisible radiation and emits visible light.

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Question 19

In a compound microscope, the lens that directs light into the eye is the (ocular/condenser/objective) lens.

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Question 20

Phage typing is useful for identifying bacteria because of the specificity of (antibodies/bacteriophages/PCR) for unique bacterial structures.

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Quiz 4: Microscopy, Staining, and Classification