You have purified a multisubunit extracellular protein that has several interchain disulfide bonds. Which of the following chemicals would you add to your purified protein mixture if you wanted to eliminate the disulfide bonds?
A) NaCl, a salt
B) SDS, an ionic detergent and denaturing agent
C) H₂O₂, an oxidizing reagent
D) Tris, a buffering agent
E) DTT, a reducing agent

Question

Quizplus · Accepted Answer

A reducing agent such as dithiothreitol (DTT) converts S-S bonds back to SH groups.

You Have Purified a Multisubunit Extracellular Protein That Has Several