How is tandem affinity purification distinct from other purification schemes such as simple affinity or ion-exchange chromatography?
A) In its use of antibodies
B) In its use of proteases
C) In its use of glutathione S-transferase
D) In its use of viral peptides
E) In its use of limited washing steps

Question

Quizplus · Accepted Answer

Since tandem affinity purification (TAP)-tagging relies on proteolytic cleavage for elution of the protein from the first column, the protein can be washed extensively at this step to remove most contaminants. Additionally, it can be purified further due to the presence of another, non-cleaved, tag.

How Is Tandem Affinity Purification Distinct from Other Purification Schemes