Which microscopy set-up uses a longer wavelength of light than usually excites a particular fluorophore? Which one allows researchers to peek deeper into biological samples?
A) Single-photon; single-photon
B) Single-photon; two-photon
C) Two photon; single photon
D) Two-photon; two-photon

Question

Quizplus · Accepted Answer

In the two-photon effect, which is employed by multiphoton microscopes, fluorescent molecules are excited almost simultaneously by two (or more) photons of longer wavelength compared to one-photon excitation. The longer wavelength provides advantages including the ability to image deep within live tissues.

Which Microscopy Set-Up Uses a Longer Wavelength of Light Than