Given the absorption and emission spectra of three fluorescent dyes in the simplified diagrams below, which pair of dyes is better suited for a fluorescence resonance energy transfer (FRET) study? Write down AB, BC, or AC as your answer.

Question

Quizplus · Accepted Answer

Good FRET pairs do not have extensive overlapping between their emission spectra or between their excitation spectra. Additionally, the emission spectrum of the donor should overlap with the excitation spectrum of the acceptor, such that Förster resonance energy transfer can be significant.

Given the Absorption and Emission Spectra of Three Fluorescent Dyes