Indicate true (T) and false (F) statements below regarding N-linked glycosylation of proteins. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
( ) N-linked glycosylation can be carried out co-translationally, possibly at multiple asparagine residues on the same protein molecule.
( ) N-linked glycosylation is a gradual process, with step-by-step addition and trimming events that commence with the addition of N-acetylglucosamine to an asparagine side chain.
( ) Most proteins synthesized in the rough ER are N-glycosylated, and some of them require this modification for their correct folding.
( ) Once a protein is properly folded in the ER, its attached oligosaccharides are quickly removed by an N-glycanase, although it may be glycosylated again later.

Question

Indicate true (T) and false (F) statements below regarding N-linked glycosylation of proteins. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
( )	N-linked glycosylation can be carried out co-translationally, possibly at multiple asparagine residues on the same protein molecule.
( )	N-linked glycosylation is a gradual process, with step-by-step addition and trimming events that commence with the addition of N-acetylglucosamine to an asparagine side chain.
( )	Most proteins synthesized in the rough ER are N-glycosylated, and some of them require this modification for their correct folding.
( )	Once a protein is properly folded in the ER, its attached oligosaccharides are quickly removed by an N-glycanase, although it may be glycosylated again later.

Quizplus · Accepted Answer

In N-linked glycosylation, the precursor oligosaccharide assembled on a dolichol anchor is transferred en bloc to certain asparagine residues in most ER proteins. Trimming and addition of further sugars then follow, and some are important for ER quality control. Correctly folded proteins usually carry oligosaccharides that are distinct from those of unfolded or misfolded proteins.

Indicate True (T) and False (F) Statements Below Regarding N-Linked