Sort the following events to reflect the order in which they occur during the formation of vesicles from the ER destined for the Golgi apparatus. Your answer would be a four-letter string composed of letters A to D only, e.g. DACB.
(A) Sar1 GTP hydrolysis
(B) Sar1 GTP binding
(C) Sar1-Sec23 binding
(D) Sar1 membrane association

Question

Sort the following events to reflect the order in which they occur during the formation of vesicles from the ER destined for the Golgi apparatus. Your answer would be a four-letter string composed of letters A to D only, e.g. DACB.
(A)	Sar1 GTP hydrolysis
(B)	Sar1 GTP binding
(C)	Sar1-Sec23 binding
(D)	Sar1 membrane association

Quizplus · Accepted Answer

A Sar1-GEF in the endoplasmic reticulum membrane activates Sar1 by guanine nucleotide exchange; Sar1 then inserts an amphiphilic helix into the membrane, one leaflet deep. Sar1 then recruits COPII coat proteins (and also a Sar1-GAP) and initiates budding. Sar1 then hydrolyzes its GTP and dissociates following a delay; however, if a sealed coat is already formed, Sar1 dissociation may not cause uncoating.

Sort the Following Events to Reflect the Order in Which