You have isolated epithelial cells from mouse mammary glands and have grown them in a three-dimensional gelatinous matrix in the presence or absence of a specific inhibitor that targets a transmembrane metalloprotease. The protease is capable of cleaving cadherin near the base of its extracellular domain to release an N-terminal fragment containing the extracellular cadherin domains. The distribution of the cells in the matrix, as seen under a light microscope, one day after the transplantation of the isolated tissue is presented in the following schematic drawings. Which condition (1 or 2) do you think corresponds to the presence of the protease inhibitor? Write down 1 or 2 as your answer.

Question

Quizplus · Accepted Answer

The inhibitor prevents the shedding of cadherin induced by the protease, and therefore facilitates cell-cell adhesion. This helps the cells clump together.

You Have Isolated Epithelial Cells from Mouse Mammary Glands and Have