In affinity chromatography
A) there is nonspecific binding of proteins to column material
B) only minor purifications can be obtained
C) the mobile phase is always pure water
D) the ligand is always specific for one type of protein to be bound
E) there can be molecule specific ligands or group specific ligands

Question

Quizplus · Accepted Answer

Affinity chromatography can use molecule-specific ligands or group-specific ligands to bind proteins, allowing for selective purification.

In Affinity Chromatography