How many bands would be produced when hemoglobin is subjected to SDS-PAGE?
A) 1
B) 2
C) 3
D) 4
E) 2, but only if the size of the pores in the gel would allow two proteins of slightly different size to be separated

Question

Quizplus · Accepted Answer

Hemoglobin is a tetrameric protein composed of two alpha and two beta subunits. When subjected to SDS-PAGE under reducing conditions, it would typically show two bands due to the separation of these subunits. However, the visibility of these two distinct bands depends on the resolving power of the gel, which is determined by the size of its pores. If the gel's pores are appropriately sized to differentiate between the slightly different sizes of the alpha and beta subunits, then two bands would be observed.

How Many Bands Would Be Produced When Hemoglobin Is Subjected