Exhibit 6A This is a reaction going on in your muscle cells right this very minute: The enzyme triose phosphate isomerase catalyzes this reaction in the forward direction as part of the glycolytic pathway. It follows simple Michaelis-Menten kinetics: Typical cellular concentrations: triose phosphate isomerase = 0.1 nM Dihydroxyacetone phosphate = 5 µM glyceraldehyde-3-phosphate = 2 µM Refer to Exhibit 6A. "Hindrate" is an inhibitor of triose phosphate isomerase. When it is added to cells at a concentration of 0.1 nM, the enzyme's K_M for the substrate is unchanged, but the apparent V_max is altered to 50 nM\sec. In the following graph, which line best represents the Lineweaver-Burk plot obtained in the presence of hindrate? A) A B) B C) C D) D E) E

Question

Quizplus · Accepted Answer

The Answer of Exhibit 6A This is a reaction going on in your muscle cells right this very minute: The enzyme triose phosphate isomerase catalyzes this reaction in the forward direction as part of the glycolytic pathway. It follows simple Michaelis-Menten kinetics: Typical cellular concentrations: triose phosphate isomerase = 0.1 nM Dihydroxyacetone phosphate = 5 µM glyceraldehyde-3-phosphate = 2 µM Refer to Exhibit 6A. "Hindrate" is an inhibitor of triose phosphate isomerase. When it is added to cells at a concentration of 0.1 nM, the enzyme's K_M for the substrate is unchanged, but the apparent V_max is altered to 50 nM\sec. In the following graph, which line best represents the Lineweaver-Burk plot obtained in the presence of hindrate? A) A B) B C) C D) D E) E

Exhibit 6A This Is a Reaction Going on in Your