Which of the following is not a function of DNA polymerase I from E. coli ?
A) adding nucleotides to the primer strand
B) 3' → 5' exonuclease activity
C) 5' → 3' exonuclease activity
D) proofreading

Question

Quizplus · Accepted Answer

DNA polymerase I from E. coli primarily functions in removing RNA primers from DNA fragments and replacing them with the appropriate nucleotides. Its main roles include 5' → 3' polymerase activity, 3' → 5' exonuclease activity for proofreading, and 5' → 3' exonuclease activity for primer removal. Adding nucleotides to the primer strand is a general function of DNA polymerases during DNA synthesis, but in the context of DNA polymerase I's specialized roles, this choice is considered not a primary function, especially when compared to its specific activities like primer removal and proofreading.

Which of the Following Is Not a Function of DNA