Sometimes DNA probes are used to prove that a gene has been incorporated into a eukaryotic genome. The following are all steps used in such a procedure, except :
A) Digestion of the cellular DNA to break it into manageable sizes.
B) Separation of the fragments by gel electrophoresis.
C) Hybridization of a nucleic acid strand complementary to the gene of interest.
D) Elution of the hybridized DNA from the gel for analysis.
E) All of these steps are necessary in probing for the presence of a gene.

Question

Quizplus · Accepted Answer

Elution of the hybridized DNA from the gel is not a necessary step in the procedure to prove gene incorporation using DNA probes. The hybridization step is typically followed by detection, not elution.

Sometimes DNA Probes Are Used to Prove That a Gene