In the polymerase chain reaction
A) it is possible to amplify small amounts of DNA without cloning.
B) conditions must be carefully controlled to prevent explosions.
C) reaction mixtures must be kept chilled at all times.
D) all of these
E) none of these

Question

Quizplus · Accepted Answer

Polymerase chain reaction (PCR) is a technique used to amplify small amounts of DNA by copying them multiple times without the need for cloning into vectors or using host cells. Conditions such as temperature are carefully controlled for denaturation, annealing, and extension phases, but not to prevent explosions or requiring constant chilling.

In the Polymerase Chain Reaction