Which of the following is a unique feature of qPCR compared to the original PCR?
A) qPCR uses a DNA polymerase from a heat stable source
B) qPCR requires a primer
C) qPCR allows the reaction to run until all of the primers have been exhausted
D) In qPCR, the speed with which the DNA is produced is used to estimate how much of the original template was in the reaction vessel

Question

Quizplus · Accepted Answer

qPCR, or quantitative PCR, uniquely allows for the quantification of DNA by measuring the amplification of DNA in real time, as opposed to the end-point detection in traditional PCR. This is achieved by using fluorescent markers to monitor the production of DNA during each cycle, thus estimating the amount of the original template.

Which of the Following Is a Unique Feature of QPCR