In producing a genomic library, human DNA and plasmid DNA must first be treated with:
A) the same restriction enzyme
B) different restriction enzymes
C) the same DNA ligase
D) different DNA ligases
E) cosmid cloning vectors

Question

Quizplus · Accepted Answer

To produce a genomic library, both human DNA and plasmid DNA must be cut with the same restriction enzyme to ensure that the ends of the human DNA fragments can properly align and ligate with the ends of the opened plasmid DNA, facilitating the insertion of human DNA into the plasmid.

In Producing a Genomic Library, Human DNA and Plasmid DNA